Fig. 1.

Mosquito infections with P. berghei and compliment responses. a P. berghei oocyst loads in the midguts of An. arabiensis (Aa), An. coluzzii (Ac) and An. quadriannulatus (Aq) pooled from three biological replicates. Horizontal red lines show the median oocyst load. Data were analysed by the Mann-Whitney test, and *** corresponds to P < 0.001. b P. berghei oocyst loads following silencing of TEP1, LRIM1 and APL1C or injection with LacZ dsRNA (control) in An. arabiensis, An. coluzzii and An. quadriannulatus pooled from two biological replicates. Horizontal red lines show the median oocyst load. Data were analysed by the Mann-Whitney test, and *** corresponds to P < 0.001. c qPCR analysis of P. berghei CSP gene abundance relative to the mosquito S7 gene IN An. arabiensis (red line), An. gambiae (black line) and An. quadriannulatus (blue line) injected with LacZ (control) or TEP1 dsRNA and infected with 10-fold serial dilutions of P. berghei ookinetes. The data show a combination of three independent biological replicates