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. 2017 Apr 19;10:191. doi: 10.1186/s13071-017-2137-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Purification of recombinant API protein and western blot. Recombinant API from the supernatants (Lane 1) and the inclusion body (Lane 2) of the bacterial cells were purified and separated by SDS-PAGE. Recombinant API was detected by serum from goat infected with H. contortus (Lane 3), and normal goat serum as negative control (Lane 4). API extracted from H. contortus was detected by rat antibody against recombinant API protein (Lane 5), and normal rat serum as negative control (Lane 6). Lane M: pre-stained protein ladder