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. 2017 Apr 19;10:191. doi: 10.1186/s13071-017-2137-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Localization of API in adult worms. Cryostal sections of H. contortus were incubated with rat antibody against the recombinant API, following with the second antibody Cy3-labeled Goat Anti-Rat IgG. The red fluorescence was detected in both female (a, d) and male (b, e) adult H. contortus, but no fluorescence was observed in control experiments (c, f). To see the distributions of the API, vertical (a, b, c) and transverse sections (d, e, f) were checked. Images in lines 1 and 2 were taken under UV light and white field; merged images are given in line 3. Scale-bars: 100 μm