Fig. 7.

Scaffold/UC-MSCs transplantation promotes regeneration of the endometrium, myometrium and blood vessels in uterine scars. a Haematoxylin and eosin (H&E) staining of uterine scars at days 30 and 60 post-transplantation in the PBS group, the scaffold group, the UC-MSCs group and the scaffold/UC-MSCs group. Arrowheads indicate repair sites. Scale bars, 300 μm. Statistical analysis of the endometrial thickness measured by Image-Pro Plus software. Statistical analysis of the number of endometrial glands per uterine cross section. b Immunohistochemical staining of α-smooth muscle actin (α-SMA) for smooth muscle abundance in uterine scars at days 30 and 60 post-transplantation in the PBS group, the scaffold group, the UC-MSCs group and the scaffold/UC-MSCs group. Arrowheads indicate repair sites. Scale bars, 300 μm. Statistical analysis of the percent of α-SMA positive areas (α-SMA-positive area in the selected region/total α-SMA-positive area) by Image-Pro Plus software. c Immunohistochemical staining of von Willebrand factor (vWF) for the blood vessel density in uterine scars at days 30 and 60 post-transplantation in the PBS group, the scaffold group, the UC-MSCs group and the scaffold/UC-MSCs group. Scale bars, 30 μm. Statistical analysis of the number of capillary vessels counted from six randomly selected fields per section under a magnification of × 400. Data were presented as mean ± SEM. ^* P < 0.05 and ^** P < 0.01. PBS phosphate-buffered saline, UC-MSCs umbilical cord-derived mesenchymal stem cells