Table 2.
Diagnostic ability of the ELISA using plasma or egg yolk samples in comparison to faecal egg counts (FEC) obtained from 24 h faeces in chickens with mono-Ascaridia galli or -Heterakis gallinarum infections
| Worm species | Parameter | ELISA - Plasma | ELISA - Egg yolk | FEC |
|---|---|---|---|---|
| A. galli | AUC ± SE | 0.99 ± 0.014a | 0.96 ± 0.034 | 0.92 ± 0.033a |
| Cut-off | 52.9 mU/ml | 32.0 mU/ml | 25 EPG | |
| Sensitivity, % (95% CI) | 94 (79–99) | 93 (78–99) | 84 (66–95) | |
| Specificity, % (95% CI) | 100 (66–100) | 100 (66–100) | 100 (66–100) | |
| H. gallinarum | AUC ± SE | 0.91 ± 0.037b | 0.96 ± 0.019b | 0.95 ± 0.023 |
| Cut-off | 47.2 mU/ml | 43.8 mU/ml | 25 EPG | |
| Sensitivity, % (95% CI) | 95 (83–99) | 90 (77–97) | 90 (77–97) | |
| Specificity, % (95% CI) | 72 (51–88) | 92 (74–99) | 100 (86–100) |
aArea under the ROC curves (AUC) sharing the superscript tend to differ between corresponding tests in A. galli infection (Chi-square test: χ 2 = 3.457, df = 1, P = 0.0630)
bAUC sharing the superscript tend to differ between corresponding tests in H. gallinarum infection (Chi-square test: χ 2 = 2.921, df = 1, P = 0.0874)