Fig. 6.

A stiff nuclear lamina prevents large deformations of the nucleus. (a) Kymographs of the nuclear envelope demonstrate that elongated cells on rectangular micropatterned islands (gray lines) have significantly smaller local perturbations of the nuclear surface compared to round cells on circular islands (red lines). These differences in the stability of the nuclear envelope are due to remodeling of the nuclear lamina, since (b) knockout of lamin A/C in elongated cells produces larger fluctuations in the nuclear surface, whereas (c) overexpression of lamin A/C in round cells has the opposite effect. Adapted with permission from Ref. [154]. Color figures are available online.