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. 2014 Sep;96(3):365–375. doi: 10.1189/jlb.2HI1013-533R

Figure 3. Macrophage polarization is unaffected in the PHD3−/− BMDM.

Figure 3.

PHD3−/− and WT BMDM, which were differentiated for 8 days, were stimulated with LPS + IFN-γ (M1 polarization) or IL-4 (M2 polarization) for 6 h; subsequently, RNA levels for the (A) M1-polarization markers IL-6, iNOS, and TNF-α and (B) the M2-polarization markers arginase, Ym-1, and FIZZ were quantified by qRT PCR. (C) PHD3−/− and WT BMDM cell culture supernatants were harvested after stimulation of the cells with LPS + IFN-γ or IL-4 for 6 h. IL-6 and TNF-α protein levels were detected by FACS, as described in Materials and Methods. (D) BMDM, which were differentiated for 5 days, were stimulated with LPS for 6 h, and TNF-α and IL-6 protein levels were determined in the cell culture supernatants; n = 3; mean ± sd; *P < 0.05, **P < 0.01, and ***P < 0.001.