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PHD3−/− and WT BMDM were (A) cultivated, with or without serum, in total for 48 h or (B) were treated with 100 μM SNAP or 500 nM stauro for 12 h. rAngptl2 was added as indicated. Induction of apoptosis was compared using Annexin V staining and FACS analysis. (C) PHD3−/− and WT BMDM were analyzed for α5β1 and PirB protein levels by FACS staining; n = 5; mean ± sd; **P < 0.01, and ***P < 0.001.
