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. 2017 Apr 19;7:46513. doi: 10.1038/srep46513

Figure 7. Effects of sub-5 SIO-Fl nanoparticles on expression of key differentiation markers and pro-inflammatory cytokines secretion.

Figure 7

(a) qPCR analysis of key differentiation markers in CaCo-2 cells treated with 10 μg/ml, 50 μg/ml, and 100 μg/ml sub-5 SIO-Fl nanoparticles after 7 days of exposure. The expression level of VIL1 and ALP1 was analysed and resulted comparable to the control ones with no significant difference. All data were expressed as the mean ± standard deviation (SD) (n = 3) (n.s., not significant). (b-d) Effects of sub-5 SIO-Fl nanoparticles on the pro-inflammatory cytokines secretion in CaCo-2 cells treated with 10 μg/ml, 50 μg/ml or 100 μg/ml of nanoparticles for 24, 48 and 72 hours. Interleukin-8 (IL-8) (b), tumor necrosis factor α (TNF-α) (c) and interleukin-6 (IL-6) (d) levels were measured in the culture supernatant by ELISA method. All data were expressed as the mean ± standard deviation (SD) (n = 4) (n.s., not significant).