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. 2017 Apr 19;7:46489. doi: 10.1038/srep46489

Figure 6. Impact of N-acetylcysteine on PEITC induction of UGT1A1.

Figure 6

N-acetylcysteine (NAC) was added to the drinking water at a concentration of 40 mM for 3 weeks. After NAC exposure, adult hUGT1 mice were treated by p.o. administration with 200 mg/kg PEITC once each day for four days. Liver was isolated after PEITC treatment. (A) From total RNA, human UGT1A1 and mouse Cyp2b10 gene expression was determined by real time PCR. (B) Using total cellular lysates, UGT1A1 expression was determined by Western blot analysis. The bands have been cropped from the full-length blots but not enhanced in anyway (S8). (C) Nqo1, Gsta1 and Gsta2 gene expression by real time PCR. Values are means ± SD (n = 3). Statistically significant differences between Vehicle (Veh) and PEITC are indicated by asterisks (Student t test: *P < 0.05)