Table 1. Content and antioxidant capacity of antioxidant compounds in O. fragrans flowers and green tea before and after the simulated digestion.
Sample | Compound | Content(mg/g DW) |
EC50(μg) |
||
---|---|---|---|---|---|
After | Before | After | Before | ||
O. fragrans flowers | Act | 10.77 ± 0.01 | 62.46 ± 0.82** | 1.42 ± 0.08 | 0.89 ± 0.05** |
IsoAct | 0.39 ± 0.00 | 2.86 ± 0.01** | 1.07 ± 0.18 | 1.46 ± 0.16* | |
ChA | 0.27 ± 0.02 | 1.19 ± 0.06* | 1.93 ± 0.15 | 0.81 ± 0.04** | |
CA | 0.08 ± 0.00 | 0.18 ± 0.00** | 2.85 ± 0.21 | 0.55 ± 0.03** | |
SAL | 1.51 ± 0.03 | 4.60 ± 0.09* | / | / | |
Longjing Tea | C | 0.54 ± 0.01 | 1.16 ± 0.01** | 1.49 ± 0.08 | 1.42 ± 0.49 |
CAF | 10. 86 ± 0.45 | 23.01 ± 0.02* | / | / | |
EC | 1.82 ± 0.04 | 7.02 ± 0.08* | 1.21 ± 0.05 | 0.88 ± 0.11** | |
ECG | 0.19 ± 0.03 | 1.78 ± 0.07** | 1.96 ± 0.09 | 0.45 ± 0.05** | |
EGC | 1.12 ± 0.05 | 3.86 ± 0.09** | 0.92 ± 0.04 | 0.51 ± 0.03* | |
EGCG | nd | 10.65 ± 0.92* | 1.44 ± 0.10 | 0.98 ± 0.04* | |
GA | 2.06 ± 0.06 | 3.76 ± 0.09** | 0.88 ± 0.07 | 1.54 ± 0.21** | |
GC | 1.14 ± 0.06 | 0.39 ± 0.05** | 2.50 ± 0.12 | 1.23 ± 0.10** | |
GCG | 0.74 ± 0.13 | 3.30 ± 0.11** | 0.99 ± 0.05 | 0.62 ± 0.06* |
Act: actoside Verbascoside; IsoAct: isoacteoside; ChA: chlorogenic acid; CA: caffeic acid; SAL: salidroside; C: catechin; CAF: caffeine; EGCG: (−)-Epigallocatechin gallate; EC: L-Epicatechin; ECG: (−)-Epicatechin gallate; EGC: (−)-Epigallocatechin; GA: gallic acid; GC: (−)-gallocatechin; GCG: (−)-Gallocatechin gallate. Values are expressed as means ± SD (n = 3). nd: Not detected.
EC50: concentration of antioxidant for 50% of maximum effect. The concentration was showed in μg for the final reaction volume (250 μL). *Value and **Value was significantly different from that after simulated digestion at P < 0.05 and P < 0.01, respectively.