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. 2017 Apr 19;7:46516. doi: 10.1038/srep46516

Figure 6. PAPLA1 regulates glycogen storage and controls the expression of carbohydrate metabolism regulators.

Figure 6

(a) PAPLA1 mutants have mis-regulated expression of genes involved in the carbohydrate metabolism. Expression of Ilp2 is reduced, whereas expression of Ilp3 increased. Expression of genes encoding enzymes involved in glycogen cleavage, tobi and Pgm, is reduced. (b) PAPLA1 mutants have increased glycogen storage. (c) Ubiquitous, adulthood-specific knockdown of PAPLA1 by daughterless-GeneSwitch (da-GS) decreases glycogen storage when the stronger RNAi line is used (i.e. line 2; see also Supplementary Fig. S1). The weaker RNAi line (line 1) has no significant effect on glycogen storage. (d) Fat body targeted, adulthood-specific PAPLA1 knockdown by FBI-26-GeneSwitch (FBI-26-GS) reduces glycogen content. (e) Fat body targeted PAPLA1 RNAi driven by Lpp-Gal4 dramatically reduces glycogen content. (f) Adulthood-specific overexpression of PAPLA1 driven ubiquitously from the daughterless-GeneSwitch (da-GS) decreases glycogen storage. (g) Adulthood-specific overexpression of PAPLA1 in the fat body, driven by the FBI-26-GeneSwitch (FBI-26-GS), decreases glycogen storage. All panels: Two-tailed Student’s t–test: **P < 0.01; ***P < 0.001.