Figure 3.

Effect of the bradykinin B₂ receptor (B₂R) antagonist on basal and lipopolysaccharide (LPS)-induced NO and tumor necrosis factor-alpha (TNF-α) release from BV2 cells. Cells were incubated with the B₂R antagonist HOE 140 (10⁻⁶ M) in the presence or absence of LPS (7 ng/ml) for 24 h. NO (A) and TNF-α (B) levels were measured in the media, and the cells were counted. Results are representatives of three independent experiments and are presented as mean ± SEM, n = 3–6; statistical significance was assessed by one-way analysis of variance followed by a Tukey–Kramer Multiple Comparison Test; ***P < 0.001 versus control.