Figure 5. Effects of wortmannin or knockdown of TtVPS34 on autophagic/lysosomal events during PND. Conjugating cells at 8 h were stained with a combination of MDC (upper) and LTR (lower). Hoechest was also used to visualize nuclei (upper panels). (A, a) Untreated wild-type cross. (B, b–E, e) Wild-type crosses treated with various concentrations of wortmannin ranging from 1 to 10 nM. (F, f) TtVPS34∆ crosses with both the parental macronuclei localized at the anterior region of the cells. (G, g) TtVPS34∆ cross with the parental macronuclei localized at middle (left) and posterior regions of the cytoplasm. White arrowheads, parental macronucleus; asterisks, developing new macronuclear anlagen. Scale bars: 10 μm.