Figure 5. LPS- or poly(I:C)-induced autophagy promotes invasion of lung cancer cells. (A and B) A549 and H460 cells were placed in the upper chamber of matrigel invasion insert, then treated with LPS (10 μg/ml) or poly(I:C) (20 μg/ml) in the presence or absence of 3MA (5 mM) for 24 h. (C and D) A549 and H460cells were transfected with control siRNA or siRNA specific for ATG5 48 h before placing in the insert. Cells that penetrated through the matrigel to the lower surface of the filter were stained, photographed (A and C; A549 cells) and cell numbers in 10 random fields were counted (B and D). Data are representative of 3 independent experiments with similar results (A and C) or are mean ± SEM of 3 independent experiments (B and D). **P < 0.01.