Figure 2.

Example section images showing the ipsilateral DG with the WGA-Cre AAV injection, and spatially restricted starter neurons in the dentate hilus in the contralateral DG. A, The AAV-mCherry-IRES-WGA-Cre strongly labels the cells (mCherry fluorescence, red) in the granule cell layer and the hilus, delineated by DAPI fluorescence (blue) in coronal sections at the septal, intermediate, and temporal levels. Scale bar, 200 μm. B, WGA-Cre activated helper AAV expression (nuclear EGFP fluorescence) is confined in the contralateral hilus of six different coronal sections at the septal to temporal levels. Arrows point to the helper AAV and rabies double-labeled starter neurons in the hilus. Scale bar, 200 μm.