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. Author manuscript; available in PMC: 2017 Apr 19.
Published in final edited form as: Cell Rep. 2017 Apr 4;19(1):1–9. doi: 10.1016/j.celrep.2017.03.026

Figure 2. ATGL overexpression is sufficient to promote autophagy/lipophagy.

Figure 2

(A) In vivo overexpression of hepatic ATGL increased autophagy target gene expression (n=5); *P<0.05 vs. Ad-Null. (B) Hepatic ATGL overexpression increased the LC3II/LC3I ratio and LAMP1 protein levels as analyzed via western blotting. Livers overexpressing ATGL had no visible bands for p62 indicative of enhanced autophagosome clearance; a representative Western blot from 3 mice is shown. (C–D) In vivo overexpression of hepatic ATGL increased LC3 and lysosomal co-localization with LDs stained with PLIN2 or lipidtox. (E) The dual reporter RFP-GFP-LC3 was transfected in primary hepatocytes that were transduced with Ad-Null or Ad-ATGL adenoviruses; (n=3 for C–E).