Table 1.

CARs chosen for this study.

Abbreviation	Source	NCBI Reference:
mpCAR	Mycobacterium phlei	WP_003889896.1
msCAR	Mycobacterium smegmatis	AFP42026.1
niCAR	Nocardia iowensis	Q6RKB1.1
noCAR	Nocardia otitidiscaviarum	WP_029928026.1
tpCAR	Tsukamurella paurometabola	WP_013126039.1

Five CARs were chosen for a thorough biochemical characterization from a range of host organisms that contain putative CARs. niCAR has been characterized previously and was chosen for comparison.11, 23 CAR abbreviations were chosen to reflect their source. NCBI ascension numbers are shown to access the protein sequences. The sequence identities of these five orthologues are provided in Table S1.