. 2017 Feb 14;9(6):1005–1017. doi: 10.1002/cctc.201601249

Table 2.

CAR activity against benzoic acid and its derivatives with electron‐donating and ‐withdrawing groups.

		Benzoic acid (1)	4‐Methylbenzoic acid (2)	4‐Methoxybenzoic acid (3)	3‐Methoxybenzoic acid (4)	2‐Methoxybenzoic acid (5)	4‐Nitrobenzoic acid (6)	3‐Nitrobenzoic acid (7)	2‐Nitrobenzoic acid (8)
Hammett		0	−0.17	−0.27	0.12	–	0.71	0.78	–
σ constants28
mpCAR	k _cat [min⁻¹]	140±20*	122±3	132±4	104±3	NA	NA	3.7±0.5	NA
	K _m [mm]	20±4*	3.7±0.2	2.8±0.2	3.0±0.2	NA	NA	0.3±0.1	NA
	k _cat/K _m [min⁻¹ mm ⁻¹]	7±1*	33±2	48±5	35±3	NA	NA	11±4	NA
msCAR	k _cat [min⁻¹]	197±4	154±6	179±6	18±1*	NA	NA	40±10	NA
	K _m [mm]	3.4±0.2	0.16±0.02	0.19±0.02	12±1*	NA	NA	0.5±0.2	NA
	k _cat/K _m [min⁻¹ mm ⁻¹]	57±4	900±100	930±80	1.4±0.2*	NA	NA	100±50	NA
tpCAR	k _cat [min⁻¹]	142±3	152±2	130±2	186±2	19±3	13±1	33±2	NA
	K _m [mm]	2.0±0.1	0.69±0.03	0.45±0.02	0.56±0.02	9±3	0.6±0.1	0.7±0.1	NA
	k _cat/K _m [min⁻¹ mm ⁻¹]	72±6	220±10	290±10	334±10	2.2±0.7	22±6	44±8	NA
noCAR	k _cat [min⁻¹]	183±6	135±5	138±4	136±5	NA	NA	59±2	NA
	K _m [mm]	2.1±0.2	1.2±0.2	1.1±0.1	0.9±0.1	NA	NA	2.5±0.3	NA
	k _cat/K _m [min⁻¹ mm ⁻¹]	89.1±8	110±20	130±10	150±10	NA	NA	24±3	NA
niCAR	k _cat [min⁻¹]	98±7	94±2	49±1	93±1	NA	NA	18±1	NA
	K _m [mm]	0.9±0.1	1.0±0.1	0.25±0.01	0.68±0.03	NA	NA	5.6±0.7	NA
	k _cat/K _m [min⁻¹ mm ⁻¹]	103±9	97±6	200±10	137±6	NA	NA	3.2±0.4	NA

NA: no activity was detected with that substrate. *: K _m was unusually large and substrate concentrations could not reach a high enough concentration to be able to determine the kinetic constants accurately. Errors represent the standard error. No Hammett constants are shown for substrates substituted in the 2‐position as steric effects cannot be accounted for properly.