Table 5.
Half‐life and degradation constants (K D) of CAR enzymes incubated at 30 °C.
| Enzyme | Half‐life | K D | TTN of | TTN of 4‐methyl‐ | TTN of 4‐methoxy‐ |
|---|---|---|---|---|---|
| [h] | [h−1] | benzoic acid | benzoic acid | benzoic acid | |
| mpCAR | 123.2 | 0.0056±0.004 | 30 000±20 000 | 20 000±20 000 | 20 000±20 000 |
| msCAR | 53.7 | 0.013±0.001 | 15 000±1000 | 10 000±1000 | 14 000±1000 |
| niCAR | 25 | 0.28±0.002 | 350±30 | 336±8 | 175±4 |
| noCAR | 35.3 | 0.02±0.002 | 9000±1000 | 6800±700 | 6900±700 |
| tpCAR | 42.9 | 0.016±0.002 | 9000±1000 | 6000±1000 | 8000±1000 |
The half‐life, K D, and TTN for the three best substrates of CAR enzymes calculated from activity after incubation at 30 °C over time, fitted to Y=Y 0×e−K*X. Standard error for K D is shown. TTN was calculated as k cat/K D, and the combined error is shown.