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. 2016 Nov 21;525(7):1599–1617. doi: 10.1002/cne.24141

Figure 2.

Figure 2

Neighbor joining tree showing the relationships of starfish relaxin‐like gonad‐stimulating peptide precursors with precursors of other members of the relaxin/insulin/insulin‐like growth factor (IGF) peptide family. The A. rubens RGP (AruRGP) precursor (blue arrow) and other starfish RGP precursors form a distinct clade within the relaxin/insulin‐like precursor family, which is highlighted in pink to distinguish it from the insulin/IGF precursor family that is highlighted in purple. A second A. rubens relaxin‐type precursor (AruRLP2; green arrow) is a paralog of the AruRGP precursor that is also positioned within the relaxin/insulin‐like clade of precursors. The full names and accession numbers of the 36 protein sequences included in the tree are as follows: AruRGP, relaxin‐like gonad‐stimulating peptide (ALJ99970.1, Asterias rubens); AamRGP, relaxin‐like gonad‐stimulating peptide precursor (BAR40315.1, Asterias amurensis); AjaRGP, relaxin‐like gonad‐stimulating peptide precursor (BAU20369.1, Aphelasterias japonica); PpeRGP, relaxin‐like gonad‐stimulating peptide precursor (BAI44654.1, Patiria pectinifera); AruRLP2, relaxin‐like peptide precursor 2 (ALJ99971.1, Asterias rubens); AplRGP, relaxin‐like gonad‐stimulating peptide precursor (LC033566.1, Acanthaster planci); PmiRGP, relaxin‐like gonad‐stimulating peptide precursor (LC057656.1, Patiria miniata); RLN1 Human, relaxin 1 precursor (NP_008842.1, Homo sapiens); RLN2 Human, relaxin 2 precursor (NP_604390.1, Homo sapiens); RLN3 Human, relaxin 3 precursor (NP_543140.1, Homo sapiens); RLN1 Mouse, relaxin 1 precursor (NP_035402.2, Mus musculus); RLN3 Mouse, relaxin 3 precursor (NP_775276.1, Mus musculus); RLN3 Alligator, relaxin 3 precursor (XP_006023546.1, Alligator sinensis); INSL3 Alligator, insulin‐like 3 (XP_006017481.1, Alligator sinensis); RLN3a Zebrafish, relaxin 3a precursor (NP_001032892.1, Danio rerio); RLN3b Zebrafish, relaxin 3b precursor (NP_001108535.1, Danio rerio); RLN3c Zebrafish, relaxin 3c precursor (NP_001108525.2, Danio rerio); INS Human, insulin precursor (NP_000198.1, Homo sapiens); INSL3 Human, insulin‐like peptide 3 precursor, (NP_005534.2, Homo sapiens); INSL4 Human, insulin‐like peptide 4 precursor (NP_002186.1, Homo sapiens); INSL5 Human, insulin‐like peptide 5 precursor (NP_005469.2, Homo sapiens); INSL6 Human, insulin‐like peptide 6 precursor (NP_009110.2, Homo sapiens); INSL3 Mouse, insulin‐like 3 precursor (NP_038592.3, Mus musculus); INSL5 Mouse, insulin‐like peptide precursor (NP_035961.1, Mus musculus); INSL6 Mouse, insulin‐like peptide precursor (NP_038782.1, Mus musculus); INSL5 Zebrafish, insulin‐like 5 precursor (NP_001122028.1, Danio rerio); INSL3 Deer, relaxin‐like peptide (AAR25542.1, Capreolus capreolus); IGF1a Human, insulin‐like growth factor 1 precursor (NP_000609.1, Homo sapiens); IGF2 Human, insulin‐like growth factor 2 precursor (NP_000603.1, Homo sapiens); IGF1 Mouse, insulin‐like growth factor 1 precursor (NP_001104745.1, Mus musculus); IGF2 Mouse, insulin‐like growth factor 2 precursor (NP_034644.2, Mus musculus); INS1 Mouse, insulin‐1 precursor (NP_032412.3, Mus musculus); INS2 Mouse, insulin‐2 precursor (NP_032413.1, Mus musculus); RLNL BRAFL, relaxin‐like peptide (EEA41967.1, Branchiostoma floridae); ILPl BRAFL, insulin‐like peptide 1 precursor ((Mita et al., 2009b), Branchiostoma floridae); ILP2 BRAFL, insulin‐like peptide 2 precursor ((Mita et al., 2009b), Branchiostoma floridae). Bootstrap values for selected nodes are shown.