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. 2016 Nov 8;35(3):611–625. doi: 10.1002/stem.2521

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© 2016 The Authors Stem Cells published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Knockout of Zeb2 impairs embryonic stem cells (ESC) neural differentiation (ND) (for general differentiation [GD], see Supporting Information Fig. S1). (A): Scheme of the 15‐day ND protocol; RA: retinoic acid. (B): RT‐qPCR of Zeb2 in Ctrl ESCs during ND. SD of two technical replicates is shown. (C): Immunohistochemistry for Zeb2 (brown) in Ctrl embryoid bodies (EBs) (Ctrl) on d6 and d15 of ND. (D‐F): Ctrl and Zeb2 KO (KO) ND‐EBs stained for Nestin (red, panel D) on d12, βIIITubulin (green, panel E) on d15 and costained for MAP2 (green) and GFAP (red) on d15 (panel F). Scale bars: 50 µm. Results shown are from one experiment and are representative for three experiments. (G) Heatmap for samples collected in pluripotency and during ND and GD with clustering based on Spearman correlation distances of quantile‐normalized RT‐qPCR values. Abbreviation: ND, neural differentiation.