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. 2017 Apr 20;2(8):e90299. doi: 10.1172/jci.insight.90299

Figure 6. Genetic inhibition of SMOC2 limits UUO-induced kidney fibrosis in mice.

Figure 6

(A) Representative Western blot (n = 5/group; Supplemental Figure 12) of αSMA, collagen 1α1, fibronectin, and SMOC2 expression using kidney samples obtained at day 7 from SMOC2-KO and WT mice subjected to UUO. (B) Representative images (n = 3/group; 5 visual fields for each tissue analyzed) of immunofluorescent αSMA staining of KO and WT kidneys from normal mice and day 7 UUO mice. Relative quantitation is represented in a box plot as arbitrary units. (C) Masson’s trichrome staining of normal and 7-day UUO kidneys from WT and KO mice. Images of Masson’s trichrome staining are representative of 5–10 visual fields for each tissue analyzed. Quantification is represented in a box plot as arbitrary units (mice n = 5–6, 5–10 visual fields/mice). Confocal images are 20× magnification; scale bar: 50 μM. Light microscopy images are 20× magnification; scale bar: 50μM. Box plots describe the median (line within box), upper and lower quartiles (bounds of box), and minimum and maximum values (bars). *P < 0.05 (WT CoK) and #P < 0.05 (WT at respective UUO) determined by one-way ANOVA with Tukey post-hoc analysis.