(A) Opposing effects of rosiglitazone and GW9662 on BACE1 expression levels. Western blot analysis was conducted at 6 hr after treatment in neurons. (B) PGC-1α effect is dependent on active PPARγ but not on its ligand. Rosiglitazone or GW9662 compounds were added to the HEK293 cells 24 hr after transfection with PGC-1α plasmid and BACE1 gene transcription was determined 6h later by qRT-PCR. Data are represented as means ± S.D. from 3 independent experiments with * indicating P< 0.05. (C) Acetylation status of PPARγ, PGC-1α and PGC-1β was determined by Western. (D) PPARγ represses BACE1 in its active, non-acetylated form. Various plasmids were transfected, alone or in combination, into HEK293 cells and BACE1 qRT-PCR was conducted 24 hr later. For panels C and D, data are represented as means ± S.D. from 3 independent experiments with * indicating P< 0.05 and ** indicating P<0.01. “n.s.” indicates non-statistically significant.