(A–C) Total RNA was extracted from three independently soil grown samples of Wild type (A), ctr1(B), and ein3 eil1
(C) genotypes 24 h after mock, JA, SA, and combined JA+SA treatments. Transcript levels of PDF1.2 were normalized to At4g34270 (T1P41-like family protein) and At4g26410 (M3E9) measured in the same samples. Data are mean ± SD of three independent biological replicates each with three technical repeats. Letters above bars indicate significant differences as determined by Tukey’s HSD (p <0.05).