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. 2017 Apr 19;12(4):e0174690. doi: 10.1371/journal.pone.0174690

Fig 4. The Sypro Ruby stained and immunoblotted SDS-PAGE gels.

Fig 4

The Sypro Ruby gel band intensities establish ZRLC content in a zebrafish adult heart extract relative to a known amount of in vitro expressed HCRLC (panel a). These samples produce calibrated blot intensities for HCRLC and ZRLC under a standardized protein immunoblotting protocol using the RLC antibody as described in Methods and previously [11]. The standards are compared to immunoblots from HCRLC-GFP and ZRLC in WT and TgGFP(+) embryos (panel b) to measure relative fractions of HCRLC-GFP and ZRLC content (ZRLCrep, Eq 1). The relative amount of ZRLC in WT and TgGFP(+) embryo hearts confirmed ZRLC content removed (ZRLCrem, Eq 2) by their comparison to control β-actin expression (panel c).