Fig 5. ELISA for MscuOBP8 detection in immature stages of Melipona scutellaris.
The native MscuOBP8 protein was detected, using an ELISA assay with a monoclonal antibody scFv anti-MscuOBP8 selected from a phage display library. The test was conducted in the larval stages (instars): L2 – 2nd stage larvae; L3 – 3rd stage (sub-stages: L3-1, L3-2 and L3-3); PDL stage (pre-defecating) and DL stage (defecating larvae); Positive control: recombinant MscuOBP8 as positive control; Negative control: carbonate/bicarbonate buffer (no protein sample).