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. 2017 Apr 19;12(4):e0175771. doi: 10.1371/journal.pone.0175771

Table 1. Identification of mixed infection by duplex ddPCR assay in comparison to real-time (performed in quadruplicate).

Sample No. Artificial Mixed of Estimated parasites concentration
(parasites/mL)
Real time PCR results Duplex ddPCR results
P. falciparum P. vivax P. malariae P. ovale P. falciparum P. vivax P. malariae P. ovale
1. PF,PV 96,460.8: 1205.76 +/+/+/+ +/+/+/+ -/-/-/- -/-/-/- +/+/+/+ +/+/+/+ -/-/-/- -/-/-/-
2. PF,PV 19,292.16: 1205.76 +/+/+/+ +/+/+/+ -/-/-/- -/-/-/- +/+/+/+ +/+/+/+ -/-/-/- -/-/-/-
3. PF,PV 3,858.43: 1,205.75 +/+/+/+ +/+/+/+ -/-/-/- -/-/-/- +/+/+/+ +/+/+/+ -/-/-/- -/-/-/-
4. PF,PV 4,823.04: 120,576 +/+/+/+ +/+/+/+ -/-/-/- -/-/-/- +/+/+/+ +/+/+/+ -/-/-/- -/-/-/-
5. PF,PV 4,823.04: 24,115.2 +/+/+/+ +/+/+/+ -/-/-/- -/-/-/- +/+/+/+ +/+/+/+ -/-/-/- -/-/-/-
6. PF,PV 4,823.04: 4,823.04 +/+/+/+ +/+/+/+ -/-/-/- -/-/-/- +/+/+/+ +/+/+/+ -/-/-/- -/-/-/-
7.* PF,PM 482,304:128,000 +/+/+/+ -/-/-/- -/-/-/- -/-/-/- +/+/+/+ -/-/-/- +/+/+/+ -/-/-/-
8.* PF,PM 96,460.8:128,000 +/+/+/+ -/-/-/- -/-/-/- -/-/-/- +/+/+/+ -/-/-/- +/+/+/+ -/-/-/-
9.* PV,PM 120,576:128,000 -/-/-/- +/+/+/+ -/-/-/- -/-/-/- -/-/-/- +/+/+/+ +/+/+/+ -/-/-/-
10. PV,PM 4,823.04:128,000 -/-/-/- +/+/+/+ +/+/+/+ -/-/-/- -/-/-/- +/+/+/+ +/+/+/+ -/-/-/-
11. PF,PO 96,460.8: 160,000 +/+/+/+ -/-/-/- -/-/-/- +/+/+/+ +/+/+/+ -/-/-/- -/-/-/- +/+/+/+
12. PV,PO 120,576: 32,000 -/-/-/- +/+/+/+ -/-/-/- +/+/+/+ -/-/-/- +/+/+/+ -/-/-/- +/+/+/+

PF: P. falciparum, PV: P. vivax, PM: P. malariae, PO: P. ovale, +: positive result,—: negative result

*The samples in which the minor species was identified by ddPCR, but not by real-time PCR