Table 1. Identification of mixed infection by duplex ddPCR assay in comparison to real-time (performed in quadruplicate).
Sample No. | Artificial Mixed of | Estimated parasites concentration (parasites/mL) |
Real time PCR results | Duplex ddPCR results | ||||||
---|---|---|---|---|---|---|---|---|---|---|
P. falciparum | P. vivax | P. malariae | P. ovale | P. falciparum | P. vivax | P. malariae | P. ovale | |||
1. | PF,PV | 96,460.8: 1205.76 | +/+/+/+ | +/+/+/+ | -/-/-/- | -/-/-/- | +/+/+/+ | +/+/+/+ | -/-/-/- | -/-/-/- |
2. | PF,PV | 19,292.16: 1205.76 | +/+/+/+ | +/+/+/+ | -/-/-/- | -/-/-/- | +/+/+/+ | +/+/+/+ | -/-/-/- | -/-/-/- |
3. | PF,PV | 3,858.43: 1,205.75 | +/+/+/+ | +/+/+/+ | -/-/-/- | -/-/-/- | +/+/+/+ | +/+/+/+ | -/-/-/- | -/-/-/- |
4. | PF,PV | 4,823.04: 120,576 | +/+/+/+ | +/+/+/+ | -/-/-/- | -/-/-/- | +/+/+/+ | +/+/+/+ | -/-/-/- | -/-/-/- |
5. | PF,PV | 4,823.04: 24,115.2 | +/+/+/+ | +/+/+/+ | -/-/-/- | -/-/-/- | +/+/+/+ | +/+/+/+ | -/-/-/- | -/-/-/- |
6. | PF,PV | 4,823.04: 4,823.04 | +/+/+/+ | +/+/+/+ | -/-/-/- | -/-/-/- | +/+/+/+ | +/+/+/+ | -/-/-/- | -/-/-/- |
7.* | PF,PM | 482,304:128,000 | +/+/+/+ | -/-/-/- | -/-/-/- | -/-/-/- | +/+/+/+ | -/-/-/- | +/+/+/+ | -/-/-/- |
8.* | PF,PM | 96,460.8:128,000 | +/+/+/+ | -/-/-/- | -/-/-/- | -/-/-/- | +/+/+/+ | -/-/-/- | +/+/+/+ | -/-/-/- |
9.* | PV,PM | 120,576:128,000 | -/-/-/- | +/+/+/+ | -/-/-/- | -/-/-/- | -/-/-/- | +/+/+/+ | +/+/+/+ | -/-/-/- |
10. | PV,PM | 4,823.04:128,000 | -/-/-/- | +/+/+/+ | +/+/+/+ | -/-/-/- | -/-/-/- | +/+/+/+ | +/+/+/+ | -/-/-/- |
11. | PF,PO | 96,460.8: 160,000 | +/+/+/+ | -/-/-/- | -/-/-/- | +/+/+/+ | +/+/+/+ | -/-/-/- | -/-/-/- | +/+/+/+ |
12. | PV,PO | 120,576: 32,000 | -/-/-/- | +/+/+/+ | -/-/-/- | +/+/+/+ | -/-/-/- | +/+/+/+ | -/-/-/- | +/+/+/+ |
PF: P. falciparum, PV: P. vivax, PM: P. malariae, PO: P. ovale, +: positive result,—: negative result
*The samples in which the minor species was identified by ddPCR, but not by real-time PCR