Fig. 4.

RSV supplementation at the diauxic shift tosir2Δ cells does not determine a short-lived phenotype. Sir2Δ cells were grown and supplied at the diauxic shift (Day 0) with RSV as in Fig. 1. (A) Survival over time of treated and untreated cultures was assessed. In the Table are shown Mean CLS and Maximum CLS values. Bar charts report Pck1 enzymatic activity (B), the ratio between Ac-K and Pck1-3HA values (C), the percentage of intracellular superoxide-accumulating cells (D), catalases/Sod enzymatic activities (E) and the levels of intracellular malondialdehyde (MDA) (F) determined for both cultures. All data refer to mean values of three independent experiments with three technical replicates each. SD is indicated.