Fig. 5.

RSV reduces the beneficial effects induced bySCH9deletion and severe CR on CLS. (A) CLS of sch9Δ and sch9Δsir2Δ cells supplemented with RSV at the diauxic shift (Day 0) as in Fig. 1. Survival of wt and sir2Δ strains is also shown for comparison. (B) The indicated strains grown as in Fig. 1, at Day 1 were transferred to water containing 100 μM RSV. Every 48 h, cultures were resuspended in fresh water and each time RSV was added. Survival of both treated and untreated cells was evaluated. In parallel, Pck1 enzymatic activity (C) and the ratio of Ac-K to Pck1-3HA values (D) were determined. All data presented are the mean values±SD of three biological replicates. Statistical significance as in Fig. 1 (**: P≤0.01).