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. 2017 Apr 19;12(4):e0175961. doi: 10.1371/journal.pone.0175961

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© 2017 Cavaliere et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — The quantitative determination of PMN chemotaxis was observed by incubating heparinized whole blood samples on leukocyte separation medium for isolation of leukocyte-rich plasma (LRP). After isolation, LRP were incubated in a water bath for 30 min at 37°C with fLMP. Values are expressed as number of PMN which have migrated by flow cytometry. (A) Data show that PMN migration induced by fMLP was comparable in XLA patients and HD. (A) After IVIg, the PMN migration remained unaltered. Histograms denote mean values and bars standard deviation. (B, C and D) An example of the FACS analysis of PMN migration of a representative XLA patient before and after IVIg infusion. The migrated cells were gated and analyzed within the quadrants of the dot plots.