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. 2017 Apr 19;12(4):e0176108. doi: 10.1371/journal.pone.0176108

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© 2017 Mercurio et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — A) Confocal laser scanning microscopy (CLSM) analyses of CXCR4 (green fluorescence) and PC-PLC (red fluorescence) localization on plasma membrane of unfixed cells. Scale bars, 18μm. B) Western blot (WB) of proteins isolated from U87MG cells by immunoprecipitation with anti-CXCR4 Ab (IP-α-CXCR4). Top panels show IP-α-CXCR4 preparations blotted with anti-PC-PLC Ab compared to control (CTRL IgG) (left) and total cell lysate (right). *IgG heavy chains. The bottom panels represent an α-CXCR4-IP preparation blotted with anti-CXCR4 Ab compared with CTRL IgG (left) and total lysate (right). C) CLSM analyses of CXCR4 (green fluorescence) and PC-PLC localization (red fluorescence) on plasma membrane of unfixed U87MG cells following the respective treatments (indicated on the left of the panel rows). Images represent untreated cells (CTRL) and cells exposed to either D609 or Plerixafor at different time-points (24h, 48h, 72h). Scale bars, 16 μm.