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. 2017 Apr 19;12(4):e0176108. doi: 10.1371/journal.pone.0176108

Fig 5. Lactate and LDH measurements in U87MG cells.

Fig 5

A) Histogram represents mean values of percentage of intracellular Lac concentration in U87MG obtained colorimetric assay after 24h, 48h, 72h of treatments. Mean value ± SD of n = 3 independent experiments. Statistical analyses were performed using one-way ANOVA. ***P = 0.0003. B) Histogram represents mean ± SD of percentage values (n = 3 independent experiments) of LDH intracellular activity measured by colorimetric assay at different treatment time-points (24h, 48h, 72h). Statistical analyses were performed using one-way ANOVA. ***P = 0.0006; unpaired two-tailed Student-t test CTRL 72h VS D609 72h P = 0.004. C) Histogram represents mean ± SD of percentage values of Lac extracellular concentration measured by a colorimetric assay on U87MG-conditioned medium in response to treatments (24h-48h-72h) (n = 3 independent experiments). All values were normalized for μg of proteins. CTRL values = 100%.