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. 2004 Dec 10;101(51):17699–17704. doi: 10.1073/pnas.0406182101

Fig. 4.

Fig. 4.

Increased sensitivity to PARP-1-dependent cytotoxicity in PARG–/– TS cells. (A and C) Microscopic analysis of PARG+/+ and PARG–/– TS cells grown with or without BZ for 3 days were examined 24 h after 5 min treatment of 0, 20, or 100 μM MNNG (A), or 0, 25, or 100 μM menadione (C). (B and D) Cell death in PARG+/+ and PARG–/– TS cells. Cells were treated with MNNG (B) or menadione (D) as described above, and quantification of total cell death was performed by using Hoechst/PI staining. Numbers on the vertical axes (A and C) depict the doses of the cell death stimuli in micromolar. (Scale bars: 25 μm.) These results (A–D) have been replicated at least three times with similar results. (B and D) *, P ≤ 0.001 between PARG–/– with or without BZ to WT with or without BZ (one-way ANOVA and unpaired Student's t test). (B and D) Error bars are shown as SEM.