Table 2. Analysis of E3.5 blastocysts from intercrosses of PARG^+/- mice.

				Hatched		Unhatched
Litters	Total blastocysts	Unhatched/total blastocysts, %	PARG+/+	+/-	ND	-/-	ND
1-3	19*	21	3	9	2	0	4
4-7	28	21	6	16	0	6	0

ND, not determined. Embryos were cultured and analyzed by light microscopy. After 72 h, embryos were genotyped by PCR. Hatched, blastocyst emerged from the zona pellucida by day 3 (Fig. 2); unhatched, blastocyst with intact zona pellucida after 3 days (Fig. 2).

^*One blastocyst was not analyzed.