Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 26;45(7):3752–3766. doi: 10.1093/nar/gkw1292

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 6. — INO80 and transcription stabilize the +1 nucleosome of CTT1. (A) Depletion of the TATA binding protein 1 (Tbp1/Spt15) from the nucleus by anchoring to the cytosolic ribosomes is rapid and prevents gene transcription. The strain HHY154 (TBP1-FRB) does not grow on medium containing 1 μg/ml rapamycin while HHY168 (TBP1 wild-type) does. (B) Nuclear depletion of Tbp1 by anchoring inhibits association of RNA Pol II to promoter and ORF of the CTT1 locus. Strains HHY168 (wild-type) and HHY154 (TBP1-FRB) were treated with rapamycin (1 μg/μl, 45 min) to anchor Tbp1-FRB to the cytosol followed by treatment with 0.4M NaCl for the indicated time points. RNA Pol II was determined by ChIP of Rpb1. (C) Tbp1/Spt15 is essential for reconstitution of promoter chromatin of the CTT1 locus after osmotic stress activation. Panels show nucleosome scan data of the CTT1 promoter and a part of the coding region (−200 to +350) of wild-type (upper panel), arp8Δ (upper middle panel), TBP1-FRB (lower middle panel) and TBP1-FRB arp8Δ (lower panel). (D) Occupancy of the CTT1 −1 nucleosome after stress treatment as determined by nucleosome scan (see Figure 4B). (E) Anchoring of Ino80 to the cytoplasm leads to reduction of CTT1 +1 nucleosome levels in unstressed cells.