Figure 4.

Translational inhibition by cycloheximide and puromycin. Untreated cells or cells treated with cycloheximide (50 μg/ml) or puromycin (50 μg/ml) for 60 min were probed with the 5΄ and 3΄ probes for Tb427.01.1740. (A) Representative Z-stack projection images from each of the three conditions. (B) Red, green and yellow spots were counted. Averages of the average values from three independent experiments are shown; in each experiment between 127 and 200 cells were analysed for each treatment. Error bars represent the standard deviation between the three different experiments.