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. 2016 Dec 7;45(7):4036–4050. doi: 10.1093/nar/gkw1203

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Knockdown activity and processing of AgoshPol47 variants. (A) The knockdown activity of the 3΄ strand on Luc-sense (upper panel) and 5΄ strand on Luc-antisense (lower panel) of the AgoshPol47 was determined by co-transfection of a luciferase reporter encoding the sense and antisense target sequence, respectively, in HEK293T cells. The regular shPol47 (21/5) was used as control. We performed three independent transfections, each in duplicate, and standard deviations were calculated. (B) Total RNA was analyzed by northern blot for processing products derived from the 3΄ strand (upper panel) and 5΄ strand (lower panel). Size markers are indicated. The regular shRNA ∼21 nt products are marked and * indicates the AgoshRNA ∼30 nt products. See the legend to Figure 2 for more details.