Figure 5.

H1 and U6 transcription efficiency of short transcripts with +1 variation. (A and D) Design of short constructs with variation at the +1 position (Human H1-N44 constructs and human U6-N44 constructs, respectively). (B and E) Total RNA was analyzed by northern blot. Size markers are indicated. AgoshPol47 and shNef were included as controls. Ethidium bromide staining of small rRNAs and tRNAs are shown as loading controls below the blot. (C and F) The RNA products were quantitated using ImageQuant. The mean values are based on three independent northern blots.