Table 1. Summary of barcode pairs and barcode-based physical clone mapping.
| Yeast S. cerevisiae | Flounder P. olivaceus | ||
|---|---|---|---|
| BAC library size | 1536 | 94 464 | |
| Barcode pair seuqencing | Clean readsa (million) | 1.5 | 30 |
| Raw barcode pairs | 52 243 | 4 315 007 | |
| Consolidated barcode pairs | 1381 (89.9%) | 90 156 (95.4%) | |
| Physical clone mapping | Pooling dimension | 3 | 5 |
| Pools | 36 | 50 | |
| Clones per pool | 96–128 | 7872–11 808 | |
| Clean readsa (million) | 0.78 | 55 | |
| Located BAC clonesb | 1324 (86.2%) | 85 173 (90.2%) | |
| Tested clones | 12 | 42 | |
| Validated clones | 12 | 42 |
aReads with QC > 20 were selected and their vector parts were trimmed.
bClones whose barcodes mapped to unique location in the BAC library by deconvolution.