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. 2016 Dec 19;45(7):4120–4130. doi: 10.1093/nar/gkw1277

Figure 5.

Figure 5.

The SLM2 response element controlling the Neurexin2 AS4 exon can operate from both upstream and further downstream of the target exon. (A) UUAA and UAAA sequence contents of wild type Neurexin2 AS4 minigene and minigenes that have the binding site cluster moved to various intronic positions upstream or downstream of the AS4 exon. The minigene insert sequence cloned into the pXJ41 exon trap vector is shown, with nucleotide numbering from the first to last nucleotide of this inserted sequence. The position of the Neurexin2 AS4 exon is indicated as a yellow pointed bar under the wild type and each mutant. The position of the 51 nucleotide SLM2 response sequence in each construct is shown as a black bar. (B) Effect of moving the UWAA cluster downstream of the Neurexin2 AS4 exon within the minigene construct, shown a bar chart. (C) Effect of moving the 51 nucleotide UWAA cluster upstream of the Neurexin2 AS4 exon on exon skipping, shown as a bar chart (data from three biological replicates). Statistical significance was addressed using t tests, and is shown on the bar charts as: ***P < 0.001; **P < 0.01; *P < 0.05. Error bars show standard error of the mean. Data previously shown in earlier panels is shown as white bars.