Table 1. Affinity measurements of the STAR domains of T-STAR and SAM68 proteins to target RNAs from Neurexin2 and Stxbp5l carried out using fluorescence polarization (FP).
| SLM2 | SAM68 | |
|---|---|---|
| Nrxn2 WT | 5.9 ± 0.9 | 2.9 ± 0.3 |
| Nrxn2 mutant 7 | 3.9 ± 0.4 | 3.4 ± 0.3 |
| UAAAAx4 | 7.9 ± 1.2 | 6.9 ± 0.8 |
| Stxbp5l 1 | 24.0 ± 5.9 | 15.5 ± 4.5 |
| Stxbp5l 2 | 18.2 ± 3.3 | 13.8 ± 2.6 |
| Poly(C) | >100 | >100 |
Poly(C) was used as a negative control. Affinities are given in Kd (μM) with standard deviations derived from three independent experiments. The RNA molecules used for FP experiments were Nrxn2 WT:
5΄-AAUUAAUUAAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA-3΄;
Nrxn2mutant7:
5΄-AAUUAAUUAAUUAAUUAACUAACCCACCCACCCUAAAAACACGAUCUUAAA-3΄;
UAAAAx4: 5΄-UAAAAUAAAAUAAAAUAAAA-3΄;
Stxbp5l 1: 5΄-ACAGUUUAAAAUUUGAUAAAAUUU-3΄;
Stxbp5l 2: 5΄-UUACAUUUAAAAGAUGAUUUAAAAA-3΄.
Poly(C): 5΄-CCCCCCCCC-3΄.