Figure 2.

Processive assay work-flow. (A) Processive assay design. Reactions are initiated by addition of Pol β, terminated with EDTA, and products ligated. Further reaction details are described in the Materials and Methods. Pol β nucleotide insertions are indicated by open boxes and by a prime designation to the respective DNA species (i.e. A to A’). (B) Representative gel of a processive reaction performed at an ionic strength of 150 mM. Time points are indicated above the lanes. The processive product is shown in red (A’B’C) and the intermediates in blue and green (A’B and B’C). (C) Representative initial velocity plot performed at 60 mM ionic strength. The products are color coded as in A and B.