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. 2017 Jan 24;45(7):3822–3832. doi: 10.1093/nar/gkx047

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Published by Oxford University Press on behalf of Nucleic Acids Research 2017.

This work is written by (a) US Government employee(s) and is in the public domain in the US.

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Figure 5. — Site-spacing dependence of F_p normalized by the insertion efficiency (E). The F_p was measured for processive substrates containing 10, 20, 40 and 80 bp spacing distances (bottom x-axis) at 150 mM ionic strength. The top x-axis represents the distance (nm) between the two 1-nt gaps. The mean and standard deviation from three independent experiments is shown. The F_p is normalized by the efficiency of insertion (Supplementary Figure S4). The red line represents the best fit to a sliding mechanism (Equation 5). The blue line is a fit to a hopping mechanism (Equation 6).