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. 2017 Feb 21;45(7):3615–3626. doi: 10.1093/nar/gkx070

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Published by Oxford University Press on behalf of Nucleic Acids Research 2017.

This work is written by (a) US Government employee(s) and is in the public domain in the US.

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Figure 1. — Plasmid sets used to measure translation initiation from non-canonical start codons. Plasmids varied in origin of replication (copy number), promoter and reporter gene characteristics. (A) Set of 64 pET20b(+) plasmids containing medium-copy pBR322 origin, T7 promoter and GFP reporter. (B) Set of 12 plasmids containing low-copy p15A origin, RhaP_BAD rhamnose-inducible native Escherichia coli promoter and GFP reporter. (C) Set of 12 plasmids containing low-copy p15A origin, RhaP_BAD rhamnose-inducible native E. coli promoter and nanoluciferase reporter. (D) Set of 12 very-low-copy bacterial artificial chromosomes (BAC) containing RhaP_BAD rhamnose-inducible native E. coli promoter and nanoluciferase reporter.