Figure 1.

DraRnl is constitutively expressed in D. radiodurans. (A) Cells were inoculated from an overnight culture into 1× TGY medium to attain A₆₀₀ of 0.05 and the culture was incubated with constant shaking at 30°C. Growth was monitored by plotting A₆₀₀ as a function of time post-inoculation. Aliquots were removed at the times specified by arrows (6, 12, 24 and 48 h). (B) Western blotting. Aliquots of cells were normalized to A₆₀₀ of 1.0, pelleted and resuspended in 10 mM sodium phosphate (pH 7.5). Cells were then lysed in SDS loading buffer, resolved by 10% SDS-PAGE, and transferred to a nitrocellulose membrane. An immunoblot with anti-DraRnl antibody is shown in the top panel. The positions and sizes (kDa) of marker polypeptides are indicated on the left. An immunoblot with anti-GlnA antibody is shown in the bottom panel.