Figure 4.

Deletion of rnl delays genome reassembly after 10 kGy of gamma irradiation. Wild-type and Δrnl cells exposed to a 10 kGy dose of gamma radiation. Aliquots of cells were removed 0, 3, 6, 9 and 12 h during the post-IR outgrowth and processed for pulsed field gel electrophoresis of NotI-digested cellular DNA as described under Experimental Procedures. Mock-irradiated control cells were analyzed in the lanes labeled –IR. A negative image of the ethidium bromide stained gel is shown. Phage λ DNA concatemers were analyzed in parallel (in lanes λ): the positions and sizes (kb) of selected λ DNAs in the ladder are indicated on the left.