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. 2016 Dec 19;45(7):4131–4141. doi: 10.1093/nar/gkw1284

Figure 2.

Figure 2

FOXO1 is a bona fide target of miR-223-3p (A) In silico prediction of miR-223-3p and FOXO1 3΄UTR mRNA interaction. (B) Each potential binding site was evaluated independently using the dual luciferase assay as presented in the ‘Materials and Methods’ section. Each dot corresponds to a biological replicate. The Renilla/Firefly ratios were normalized on paired to miR-Ctrl conditions (Norm. Lucif.). Data represent the quantification of three biological replicates, ‘centre values’ as mean and error bars as s.d. * for P < 0.05. (C) FOXO1 expression in A549 cells after 48 h transfection with miR-223-3p. (D) FOXO1 expression in A549 cells co-cultured overnight with PMN. (C and D) FOXO1 expression was analysed by immunoblotting. β-ACTIN served as reference for loading.