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. 2016 Dec 6;45(7):4068–4080. doi: 10.1093/nar/gkw1225

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Full-length Ntr1 is sufficient to promote dissociation of the ILS by Prp43. 10–30% glycerol gradient sedimentation of purified ILS incubated in solution with (A) ATP plus NTR, (B) no recombinant protein, (C) Prp43 and full-length Ntr1 (Ntr1FL), (D) Prp43 and Ntr1 G-patch motif (Ntr1GP), (E) Prp43, Ntr1GP and Ntr2. U2, U5 and U6 snRNAs were visualized by northern blotting followed by autoradiography. RNA identities are indicated on the left. Quantifications were performed with ImageQuant software (Molecular Dynamics). ILS: numbers represent the percentage of intron-lariat RNA released in the peak fractions (sum of fractions 3–7) or associated with the ILS (unreleased, sum of fractions 13–17) relative to the intron-lariat RNA distributed in fractions 3–7 and 13–17, the sum of which was set to 100%. U2 and U5: numbers represent the percentage of U2 or U5 snRNAs released in the peak fractions (sum of fractions 8–12) or associated with the ILS (unreleased, sum of fractions 13–17) relative to the U2 or U5 snRNA distributed in the fractions 8–12 and 13–17, the sum of which was set to 100%. U6: numbers represent the percentage of U6 snRNA released in the peak fractions (sum of fractions 2–6) or associated with the ILS (unreleased, sum of fractions 13–17) relative to the U6 snRNA distributed in the fractions 2–6 and 13–17, the sum of which was set to 100%.