Table 1.
Fertilization Outcomes with Dried Sperm Organized by Species, Drying Method, and Storage Conditions, Including Temperature and Duration of Storage
Species | Method | Tstorage (°C) | Duration | % Blastocyst | Pregnancy outcome | Reference no. |
---|---|---|---|---|---|---|
Mouse | CD | 4 | 18–24 Hours | 64 | 11 Fetuses on day 15 from 85 embryos | 10 |
4 | 1 Month | 63 | 3 Pups from 40 embryos (7.5%) | 78 | ||
4 | 1 Year | 30.4 | 2 Pups from 38 embryos (5%) | 81 | ||
22 | 3 Months | 12.2 | 2 Pups from 59 embryos (3%) | |||
4 | 2 Years | 41.4 | 82 | |||
22 | 2 Years | 3 Pups from 51 embryos (6%) | ||||
FD | 4 | 9 Pups from 32 embryos (21%) | 85 | |||
25 | 16 Pups from 86 embryos (19%) | |||||
4 | 6 Months | 13 | 40 | |||
4 | 6 Months | 50 | 79 | |||
4 | 5 Months | 9 Pups from 43 embryos (21%) | 80 | |||
4 | 1 Year | 22 Fetuses on day 15 from 60 embryos | 83 | |||
4 | 1.5 Years | 15 Pups from 72 embryos (21%) | ||||
4 | 3 Years | 17 Pups from 92 embryos (47%) | 84 | |||
Rat | FD | 4 | 1 Year | 7 Pups from 102 embryos (7%) | 86 | |
4 | 1 Year | 3 Pups from 19 embryos (16%) | 88 | |||
4 | 5 Years | 10 Pups from 92 embryos (11%) | 89 | |||
Cat | FD | 4 | <72 Hours | 27.9 | 90 | |
Rabbit | 4 | 12–24 Months | 24 | 1 Dead kit from 230 embryos (0.4%) | 91 | |
Horse | 4 | 3.5 Months | 5 Pregnancies from 7 embryos, 2 foals from 3 gestations | 92 | ||
Bull | 4 | 1–3 Months | 29.6 | 93 | ||
CD + heat | 4 | 1 Month | 14 | 94 | ||
25 | 7–10 Days | 5 | ||||
Pig | FD | 4 | 1 Month | 10.7 | 95 | |
Monkey | 25 | 1–2 Months | 25 | 96 |
CD, convective drying; FD, freeze-drying.