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. 2017 Apr 1;15(2):158–168. doi: 10.1089/bio.2016.0087

Table 1.

Fertilization Outcomes with Dried Sperm Organized by Species, Drying Method, and Storage Conditions, Including Temperature and Duration of Storage

Species Method Tstorage (°C) Duration % Blastocyst Pregnancy outcome Reference no.
Mouse CD 4 18–24 Hours 64 11 Fetuses on day 15 from 85 embryos 10
    4 1 Month 63 3 Pups from 40 embryos (7.5%) 78
    4 1 Year 30.4 2 Pups from 38 embryos (5%) 81
    22 3 Months 12.2 2 Pups from 59 embryos (3%)  
    4 2 Years 41.4   82
    22 2 Years   3 Pups from 51 embryos (6%)  
  FD 4     9 Pups from 32 embryos (21%) 85
    25     16 Pups from 86 embryos (19%)  
    4 6 Months 13   40
    4 6 Months 50   79
    4 5 Months   9 Pups from 43 embryos (21%) 80
    4 1 Year   22 Fetuses on day 15 from 60 embryos 83
    4 1.5 Years   15 Pups from 72 embryos (21%)  
    4 3 Years   17 Pups from 92 embryos (47%) 84
Rat FD 4 1 Year   7 Pups from 102 embryos (7%) 86
    4 1 Year   3 Pups from 19 embryos (16%) 88
    4 5 Years   10 Pups from 92 embryos (11%) 89
Cat FD 4 <72 Hours 27.9   90
Rabbit   4 12–24 Months 24 1 Dead kit from 230 embryos (0.4%) 91
Horse   4 3.5 Months   5 Pregnancies from 7 embryos, 2 foals from 3 gestations 92
Bull   4 1–3 Months 29.6   93
  CD + heat 4 1 Month 14   94
    25 7–10 Days 5    
Pig FD 4 1 Month 10.7   95
Monkey   25 1–2 Months 25   96

CD, convective drying; FD, freeze-drying.